Regulation of Bacteriophage T5 Development by ColI Factors
نویسندگان
چکیده
منابع مشابه
Polarized injection of the bacteriophage T5 chromosome.
Examination of the first-step-transfer DNA of T5ris mutants which carry new EcoRI sites showed that the left end of the chromosome is injected first.
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Bacteriophage T5 has a 120 kb double-stranded linear DNA genome encoding most of the genes required for its own replication. This lytic bacteriophage has a burst size of ∼500 new phage particles per infected cell, demonstrating that it is able to turn each infected bacterium into a highly efficient DNA manufacturing machine. To begin to understand DNA replication in this prodigious bacteriophag...
متن کاملGenetic suppressors of bacteriophage t5 amber mutants.
Nonsense mutations in structural genes appear to abort polypeptide chain synthesis at the amino acid site governed by the mutant codon. The two presently recognized types of nonsense mutant, amber and ochre, have been assigned the codons UAG and UAA, respectively. Certain bacteria harbor one or more genetic suppressors that counteract the effect of appropriate nonsense mutations in their own st...
متن کاملFluorescence changes of a membrane-bound dye during bacteriophage T5 infection of Escherichia coli.
The fluorescence intensity of membrane-bound N-phenyl-1-naphthylamine increases dramatically when T5 bacteriophage infect colicin Ib plasmid-containing hosts. This dramatic increase is not seen during normal infections or in infections wherein either the plasmid or the phage contain mutations which allow productive infection to occur. Two smaller increases in fluorescence intensity are seen, ho...
متن کاملPurification and properties of a bacteriophage T5-modified form of Escherichia coli RNA polymerase.
A modified form of Escherichia coli RNA polymerase that contains one of the three T5-specific polypeptides known to interact with the host enzyme was purified from bacteriophage T5-infected cells. The properties of this T5-modified enzyme appeared identical to those of the RNA polymerase derived from uninfected non-colicinogenic cells and to a fully active enzyme isolated from T5-infected ColIb...
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ژورنال
عنوان ژورنال: Journal of Virology
سال: 1972
ISSN: 0022-538X,1098-5514
DOI: 10.1128/jvi.9.5.804-812.1972